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人高敏型心肌肌鈣蛋白I(hs-cTnI)酶聯(lián)免疫吸附檢測(cè)試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK9080

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00
96T*5	￥13600.00

下載說(shuō)明 ①

Overview

Product name:	Human hs-cTnI (High Sensitivity Cardiac Troponin I) ELISA Kit
Reactivity:	Human
Alternative Names:	High Sensitivity Cardiac Troponin I
Assay Type:	Sandwich
Sensitivity:	9.9 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cardiovascular biology
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-cTnI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-cTnI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-cTnI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-cTnI in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.219	2.132
1000.00	1.703	1.616
500.00	1.174	1.087
250.00	0.847	0.760
125.00	0.559	0.472
62.50	0.361	0.274
31.25	0.216	0.129
0.00	0.087	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant hs-cTnI and the recovery rates were calculated by comparing the measured value to the expected amount of hs-cTnI in samples.

Matrix	Recovery range	Average
serum(n=5)	79-93%	86%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	88-102%	95%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-cTnI and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.